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As described earlier, dextromethorphan is an uncompetitive NMDA receptor antagonist and sigma-1 receptor agonist that is quickly metabolized [21]

A CellProfiler pipeline was designed to detect and count live and dead cells, mitochondria and lysosomes from immunofluorescence images using automatic thresholding and segmentation methods (see All experiments were repeated at least 2-3 times with different inductions (hiPSC derived neurons)